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教師簡介 教學大綱 課程內容 研究方向

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教師簡介

　朱大成 助理教授

Da-Chang Chu, Assistant Professor

Ph.D. in Medical Genetics (Univ. of Alabama at Birmingham, 1996)

Tel: 886-3-328-3016X5086

Fax: 886-3-328-0174

E-mail: dcchu@mail.cgu.edu.tw

　

Selected recent publications

　

1. Da-Chang Chu, Sara C. Finley, Daniel W. Young, and Virginia K. Proud (1997). CNS malformation in a child with Kabuki (Niikawa-Kuroki) syndrome: report and review. Am J Med Genet 72:205-209.

2. Wenstrom KD, Owen J, Chu DC, and Boots LR (1997). Alphafetoprotein, free beta human chorionic gonadotropin, and dimeric inhibin A produce the best results in a three analyte multiple marker screening test for fetal Down syndrome. Am J Obstet Gynecol 177:987-991.

3. Wenstrom KD, Owen J, Chu DC, and Boots LR (1997). Free beta hCG subunit versus intact hCG in the multiple marker screening test for fetal Down syndrome. Obstet Gynecol 90:370-374.

4. Wenstrom KD, Owen J, Chu DC, and Boots LR (1997). Elevated second trimester dimeric inhibin A levels identify Down syndrome pregnancies. Am J Obstet Gynecol 177:992-997.

5. Chu DC, Hsu C, Wenstrom KD, and LR Boots (1998). Insulin-like growth factor binding protein-3 in the detection of fetal Down syndrome pregnancies. Obstet Gynecol 91:192-5.

　

　

　

　

教學大綱

1. Molecular and cytogenetic diagnostic techniques

2. Cell biology

3. Molecular biology

4. Biotechnology experiment

5. Research methodology in medical technology

課程內容

1. Molecular and cytogenetic diagnostic techniques

2. Cell biology

3. Molecular biology

4. Biotechnology experiment

5. Research methodology in medical technology

　

　

研究方向

　

Research Interests

研究內容:
  1. Prenatal screening / diagnosis of fetal Down syndrome

Down syndrome is the most common chromosomal aneuploidy observed in live births. The incidence has been reported to be 1 in 1000. Clinical findings include characteristic facial features, mental retardation, cardiovascular anomalies, respiratory system abnormalities, and gastrointestinal malformations. It has been one of the major concern in prenatal care.

We plan to study the DNA polymorphism on 3 human chromosome 21-specific PCR markers and determine the number of chromosome 21 in the fetal cells. These 3 loci contain unique tetranucleotide repeats. With primers designed specifically for each locus, the repeat sequences can be amplified and distinguished by polyacylamide electrophoresis.
  1. 唐氏症之產前篩檢及診斷

唐氏症是最常發生於活產新生兒之染色體異常，其發生率約為千分之一，因此被列為優生保健之重點項目。唐氏症乃導因於生殖細胞進行減數分裂時第廿一號染色體發生無分離現象(Nondisjunction)，因而產生二倍數（diploid）之精（卵）子。當其與正常的單倍數（Haploid）卵（精）子結合時，就產生第廿一號染色體三體現象。

本研究室擬以PCR來直接大量複製羊水細胞中第廿一號染色體上的特異Tetranucleotide 重覆序列，再利用其多樣性（Polymorphism）以凝膠電泳和化學螢光呈色偵測法來判定細胞內的廿一號染色體數目（正常者有二條帶，而唐氏症有三條帶），藉以診斷唐氏症。

B. To establish molecular diagnosis laboratory

乙、建立分子診斷中心

　

　Last updated: 03/20/99